System Features:

• Open frame system
• Automatic operation
• Well-designed system for Indian conditions
• Microprocessor based PLC control/ independent control system
• Insitu or autoclavable system
• Auto or manual sterilization facility
• PLC/ SCADA control facility
• Vessel SS 316 or glass
• Electroplated vessels
• User-friendly software
• pH/DO/Foam control
• PO2 / redox controller and monitoring
• Cascade / multiple control facility
• Data logging facility
• Provision for scaling up from lab scale to pilot and process scale
• Top motor drive or bottom magnetic drives with mechanical seal
• Temperature control system
• Automatic/manual sample feed facility
• Optional facility for analysis of Glucose (online

External jacket

with cladding insulation is provided for water circulation. Jackets are of SS 304 with a polish bright finish.

Agitation

Agitation is provided using the top motor drive with mechanical or with a special optional bottom motor drive, which is a direct drive to the impeller shaft. The magnetic drives are optionally available. Electronic speed controllers are microprocessor-based systems with digital display and speed range from 10rmp to 1000rmp

Flexible Stirrer System has

• Slow stirrer speed using a reduction gearbox for maximum stability at low rpm
• Robust stainless steel framework
• Small footprint
• Stainless steel drip tray pulls out for easy cleaning and improved vessel handling

• Easy to clean and maintain. Each module is totally independent, using its own power supply, pumps etc. Although individual, the modules can stack together to create an equipment familiarization time may be limited. Because the modules are totally independent, they can also easily be used to up-grade older equipment.

Temperature Controller permanently displays temperature on an LED display and, at the touch of a bottom displays the set point. The temperature controller uses low voltage heaters available in a wide range of sizes and shapes to suit individual vessels. Sensors are individually calibrated for accurate temperature measurement.

pH Controller after temperature, pH is the most relevant parameter to measure and control. The 260 features high impedance integrated amplifier to ensure accurate measurement of pH and to enable a wide range of pH electrodes to be used. The pH controller uses the industry standard peristaltic pumps.

Oxygen Meter (DO controller) is able to measure dissolved air/oxygen in solution (DO). It has two built-in pre-amplifiers to enable it to cope with either the low-cost glass galvanic electrode or the more expensive stainless steel polarographic electrode.

The Stirrer is constructed using a durable stainless steel framework and the powerful top drive motor is of the highest specification to give maximum performance. The vessels are of tough borosilicate glasses, which are designed for sterilization in an autoclave. The stirrer is fitted with a reduction gearbox, which provides constant low speeds and also protects the motor against wear and tear.

The whole assembly provides easy access for removal of vessel, inner parts and drip tray, whilst providing a useful stainless steel back plate for mounting additional items such as air control valves and water pipe work.

The Controller is simple to use, powerful and effective. It uses a clear fluorescent display, showing all key parameters on one screen at the same time. A single keystroke takes you into specific calibration screens for each parameter, keeping the display uncluttered and intuitive.

Built-in pumps and optional pump module efficiently tackle both batch and continuous flow fermentation

Glassware is profiled with a rounded bottom to minimize cell shearing and a low-density heater provides essential uniform heating.

Low shear, variable pitched impellers can be designed to your own specification in order to achieve maximum cell growth. Combine these features with DO and pH control and the Cell Culture System will tackle any cell line.

Comprehensive Measurement & Control System has

• Microprocessor controlled
• Key information is clearly displayed on one screen
• Single stroke access to individual parameters
• Plug and Go" additional signal conditioners for easy system expansion
• Full access to PID values
• Links to purpose built software packages for data logging and control of up to four units
• Large low-wattage heater for good heat distribution The Right Vessel for your Fermentation, yes as we provide
• Specifically designed dish-based vessel with low specific ratio
• Low shear impellers with variable pitch
• Large range of vessels from 0.5 to 10 liter working volumes

• Vessel skirt allows vessel to stand upright for ease of handling Gas Flow System we offer DO control by blending oxygen/air and nitrogen pH control by CO₂ valve and flow meter, or with standard pump. Separate flow meters for O₂, N₂, CO₂ and air are provided

Mammalian Cell Culture

New milestone has been reached in biotechnology that brings complete design flexibility and simplicity to the production of mammalian cells and their byproducts. It is now possible to use a variety of cell culture methodologies for the successful growth of virtually all eukaryotic cell lines.

We offer an universal system has been designed for the growth of anchorage-dependent and suspension cultures. For secreted products a fibrous-bed of polyester disks may be employed as a matrix for high-density growth of cells immobilized on the disks. This support is ideal for production of secreted proteins regardless of cell type. Whether growing insect cells, hybridomas or recombinant anchorage-dependent cells, the fibrous-bed concept can maximize productivity. For some suspension cell lines, marine and pitched blade impellers may be preferred to minimize shear forces and enhance O₂ transfer rate ( eg. double screen-impeller increases O₂ transfer by 20%).

System Features:

• Interactive 4-gas control with sequential addition of air, O₂, N₂ and CO₂ for precise pH and D.O. control.
• Internal ring sparger and air/gas overlay facilities.
• Five programmable pumps with assignable functions for feed/harvest, level/antifoam, acid and base addition.
• Computer interface for data logging and computer control.
• Drive motor easily uncoupled for removal of vessel.
• 4-channel recorder output for pH, D.O., temperature and stirring speed.
• Eliminates need for micro carriers with anchorage-dependent cells.
• Entrapped cells avoid shear from impellers, gas bubbles and pumps.
• Cell entrapment allows higher mixing speeds, increasing mass transfer of nutrients and oxygen.
• Capable of continuous measurement of oxygen uptake rate for real-time cell mass determination.
• Secreted products automatically separated from cells in perfusion, eliminating filtration and membrane fouling. Simplified scale-up from 1.4 liters to custom-designed production systems.

Technical Specifications:

Vessel material: glass for sizes 1 to 10lits; stainless steel (SS316L) for sizes from 1 to 2000liters

Standard capacity: 1,2,5,10,15, 20,30,40,50, & 100 lit. And above or as per requirement

Working volume: aprox. 80%

Top plate: 6-12port with SS 316 and side port 4-6nos.

Agitation: electric motor (1 HP/0.5 HP) with top drive

Magnetic drive and bottom drives are also available as optional Speed control: electronic control variable speed/range as per application (10-1000rpm)

Motor: Ac/DC motor 0.5 HP or 1 HP or higher depending on application

Temperature: PID controller with display; Heating /cooling water from external water bath

pH control: autoclavable electrodes/insitu sterilizable with PID controller with display range 2-14 Accuracy 0.1 pH

DO control: ranges 0-100% saturation

Antifoam control: as per requirement

Dosing systems: peristaltic pumps for acid, alkali, and feed/fed batch/continuous system provision

PLC/PC: provided with provision for output alarms

Sterilization: autoclavable (up to 20lit) insitu sterilization (auto/manual)

Mounting frame: table top/open frame

Software: Data acquisition and reporting software with graphics. Advanced control software for multifermentor system based on SCADA

Computer: Pentium 4 or better with color monitor and printer

Certain System Components

• Reactor vessel
• Jacket
• Insulation
• Inoculum connection
• Ports for pH, temp, dissolved O₂ sensor
• Agitator
• Gas Sparger
• Mechanical seals
• Reducing gearbox
• Motor
• Harvest nozzle
• Jacket connection
• Sample valve with steam connection
• Sight glass
• Connection for acid, alkali and antifoam chemicals
• Air inlet
• Removal top
• Removal or feed nozzle
• Instrument ports
• Foam breaker
• Sight glass
• Steam connection (port)
• Rupture disc nozzle